Fig. 3. The up-regulation of LINC00662 expression facilitated proliferation, migration, invasion, and EMT of OSCC cells by inhibiting miR-144-3p expression. (A) qRT-PCR was used to detect the expression of miR-144-3p in OSCC cells transfected with LINC00662 overexpression plasmid or co-transfected with LINC00662 overexpression plasmid and miR-144-3p mimics, respectively. (B) The proliferation of transfected cells was detected by CCK-8 method. (C and D) Transwell experiment was used to detect the migration and invasion of transfected cells. (E) Protein expression levels of E-cadherin and Vimentin in transfected cells were detected by Western blot. ^*p<0.05, ^**p<0.01, ^***p<0.001. EMT, epithelial-mesenchymal transition; qRT-PCR, quantitative real-time polymerase chain reaction; OSCC, oral squamous cell carcinoma; CCK-8, Cell counting kit-8.