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. 2021 Jun 28;7:56. doi: 10.1186/s40729-021-00341-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — ALP staining images of BMSCs cultured on SLA surfaces (a) and SLA discs treated with 20 mM (b), 50 mM (c), 100 mM (d), or 200 mM (e) sodium bicarbonate after cell osteogenic differentiation for 7 days. Quantitative results of the relative activity of ALP in BMSCs on different surfaces after 7 days (f). Data are presented as the mean ± standard deviation (n = 3, *P < 0.05; **P < 0.01; ***P < 0.001)