Fig. 2.

Dose validation study with 5 mg/kg intravenous melphalan. Melphalan caused transient weight loss (a–b) and hypocitrullinemia (c), with a strong correlation between melphalan-induced weight loss and citrulline (d). Melphalan caused anorexia (e) but did not affect water intake (f). Body temperature spiked 6 days post-melphalan (g) coinciding with white blood cell nadir (h–i). Data represented as individual values (a, d), mean ± SEM (b–h, excluding d) or stacked means (i). Simple linear regression analysis (± 95% CI) with Pearson’s correlation was performed for all association plots