Fig. 3.

Five mg/kg intravenous melphalan causes severe anatomic derangement which correlates with plasma citrulline. Villus length (a, b) and crypt depth (c–e) were assessed at day 4, 7 and 10 and were correlated with matched plasma citrulline values (f–g). Panel h shows representative images of intestinal architecture. Panel i shows colonic perforation observed in one rat 7 days post-melphalan, with areas of frank ulceration (arrow) and total destruction of mucosa, submucosa and muscularis (i ii/iii). Data presented as mean ± SEM (a–e). All scale bars show 250um with the exception of panel i (ii) which shows 1 mm. Simple linear regression analyses (± 95% CI) with Pearson’s correlation analysis was performed for all association plots