Extended Data Fig. 2. Both male and female adult mice exhibit social reward behavior.

a, b, Preference for social port and numbers of pokes in females (a) and males (b) in the presence of a target animal. Both male and female animals develop strong preference for the social port in the presence of target animals. Experiment was performed over a 10-day period, which consists of 1 day of baseline session, 7 days of training sessions with target animals, and 2 days of post-training sessions. c, d, Preference for social port and numbers of pokes in females (c) and males (d) in the absence of a target animal. Neither male nor female animals develop preference for the social port. Experiment was performed over an 8-day period, which consists of 1 day of baseline session and 7 days of training sessions without target animals. e, Percentage of male and female mice exhibiting consistent preference for social port (defined as showing >20% preference on both days 6 and 7). In (a, b, e), with target animals, n = 17 females and 20 males; these include 9 females and 8 males presented in Fig. 1 as well as an additional 8 females and 12 males presented in Extended Data Fig. 1. In (c, d, e), without target animals, n = 15 females and 10 males. In (a-d), left panels, one-way repeated measures ANOVA with Bonferroni post-hoc correction (*P < 0.05, **P < 0.01, ***P < 0.001); right panels, two-way repeated measures ANOVA with Bonferroni post-hoc correction (**P < 0.01, ***P < 0.001). (a-d), mean ± SEM. For detailed statistics information, see Supplementary Table 1.