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. 2021 Jun 16;32(11-12):589–598. doi: 10.1089/hum.2020.175

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© Yoshie Kurokawa et al., 2021; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Filipin staining of each mouse liver. The accumulation of unesterified cholesterol was detected in the liver of untreated Npc1^−/− mice (A) but not detected in that of AAV-Npc1^+/+ mice (C). The liver of AAV-Npc1^−/− mice (B) was also positive but showed a weaker signal than that of untreated Npc1^−/− mice. Scale bar = 50 μm (A–C).