FIG. 2.

Validation of a PQ toxicity assay. (A) Cell count of Jurkat cells exposed to a titration of PQ over a 15-day time course. Error bars represent SEM for three technical replicates. (B) Left: Clonal expansion of Jurkat cells in PQ normalized to the untreated condition. Right: Viability of Jurkat cells in PQ. Measurements after 3 days of exposure. Error bars represent SEM for three technical replicates. (C) Competition assay of either SOD1 KO cells or NT control cells cultured with wild-type cells in the presence of PQ. This is representative of three experiments, each performed with three SOD1 sgRNAs, which all had a consistent phenotype. NT, nontargeting; PQ, paraquat; SEM, standard error of the mean.