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. 2021 Jun 14;8:662312. doi: 10.3389/fmed.2021.662312

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Copyright © 2021 Patel, Hissong, Rosado, Burkhardt, Cong, Alperstein, Siddiqui, Park, Song, Velu, Rennert and Heymann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Percentage of nucleated cells that are tumor cells as estimated using formalin-fixed, paraffin-embedded cell block sections vs. using a ThinPrep® slide. (A) Three cases (orange oval), all of which demonstrated low overall cellularity on a ThinPrep® slide also demonstrated discordant tumor percentage estimation. (B) When the three outlier cases are removed, cell block and ThinPrep® tumor percentages demonstrate a strong and statistically significant linear association. An example of one of the three outlier cases demonstrates an exclusive population of tumor cells (C) on the ThinPrep® slide (Papanicolaou stain, 600x), while clusters of tumor cells are intermixed with numerous histiocytes and inflammatory cells (D) on cell block sections (hematoxylin and eosin, 600x).