TABLE 3.
Percentage of sperm cells with DNA damage after hydrogen peroxide and DNAse treatments in different proteinase K conditions.
| Percentage of positive cells |
|||
| Proteinase K 0 min | Proteinase K 30 min | Proteinase K 180 min | |
| Fresh – alkaline comet | |||
| Control | 30 ± 22% | 35 ± 36% | 15 ± 24% |
| H2O2 0.01% | 71 ± 29%a | 80 ± 23%a | 64 ± 23%a |
| H2O2 0.1% | 86 ± 18%a | 69 ± 35% | 85 ± 11%a |
| DNAse I – 1U | 67 ± 17%a | 62 ± 42% | 46 ± 30% |
| DNAse I – 4U | 74 ± 26%a | 64 ± 33% | 45 ± 31% |
| Fresh – neutral comet | |||
| Control | 6 ± 12% | 14 ± 13% | 1 ± 3% |
| H2O2 0.01% | 0 ± 0% | 23 ± 16%* | 4 ± 4% ¶ |
| H2O2 0.1% | 0 ± 1% | 18 ± 4%* | 3 ± 4% ¶ |
| DNAse I – 1U | 70 ± 41%a | 56 ± 19%a | 68 ± 25%a |
| DNAse I – 4U | 63 ± 25%a | 84 ± 32%a | 71 ± 25%a |
| Cryopreserved – alkaline comet | |||
| Control | 62 ± 22% | 48 ± 21% | 17 ± 20%* |
| H2O2 0.01% | 94 ± 8% | 93 ± 13%a | 48 ± 47% |
| H2O2 0.1% | 100 ± 0% | 93 ± 6% a | 52 ± 13%a,¶, * |
| DNAse I – 1U | 50 ± 32% | 26 ± 13% | 14 ± 12% |
| DNAse I – 4U | 73 ± 22% | 34 ± 19%* | 17 ± 7%*¶ |
| Cryopreserved – neutral comet | |||
| Control | 0 ± 0% | 19 ± 9%* | 0 ± 1%¶ |
| H2O2 0.01% | 0 ± 0% | 19 ± 7%* | 3 ± 3% ¶ |
| H2O2 0.1% | 0 ± 0% | 15 ± 1%* | 20 ± 20% |
| DNAse I – 1U | 9 ± 15% | 70 ± 27%a,* | 97 ± 5%a,* |
| DNAse I – 4U | 50 ± 50% | 83 ± 26%a | 99 ± 1% a |
*Differences compared to 0 min of proteinase K in similar treatment (p < 0.05). ¶Differences compared to 30 min of proteinase K in similar treatment (p < 0.05). aDifferences compared to control in similar proteinase K treatment (p < 0.05).