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. 2021 Jun 14;9:646077. doi: 10.3389/fcell.2021.646077

FIGURE 4.

FIGURE 4

WASp is involved in F-actin accumulation in the GCB contact zone. (A,B) GCBs isolated from SRBC-immunized WT and WKO mice were incubated with Fab’-PLB, fixed, permeabilized, stained for F-actin with phalloidin, and imaged using IRM and TIRF. Shown are representative images (A) and the MFI of phalloidin in the GCB contact zone at different times (B). (C–E) GCBs isolated from SRBC-immunized WT and WKO mice expressing Lifeact-GFP were incubated with Fab’-PLB and imaged live using TIRF. Shown are representative time-lapse TIRF images of Lifeact-GFP (C), the MFI of Lifeact-GFP in the contact zone over time (D), and the increasing rates of Lifeact-GFP MFI, measured as illustrated in Figure 2E. Data points represent individual cells. Scale bars, 3 μm. The results were the average (± SD) of 22∼50 individual cells per condition. n = 4. **p < 0.01, ***p < 0.001, and ****p < 0.0001, unpaired Student’s t-test or one-way ANOVA.