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Example of a Z-stack acquisition in a patient with oropharyngeal carcinoma. (A) Definition of the first focus plane, here 111 µm below the mucosa surface of the tumor. (B–AI) Automated recording of the Z-stack in both directions (to surface and depth) relative to the current focal plane. The Z step, i.e. the interval of depth between the individual images, was set at 3 μm. CLE image size: 464 × 261 µm, 1,920 × 1,080 pixel.
