FIGURE 2.

SRP14 and HMGB3 knockdown impact HIV latent and productive infection. (A) Short-list of 13 proteins chosen for follow-up validation and two control proteins, PTB and HSP90A that are known regulators of HIV-1 replication. (B) Genome of the single round DuoFluo virus [R7/E-/GFP-EF1α-mTagBFP2 (R7GEmTB)] used in this study. The fluorescence profiles that correspond to latent or productive infection are shown in the grid. (C) Bulk populations of RFP⁺ shRNA knockdown (KD) Jurkats were infected with the single round DuoFluo virus pseudotyped with the dual-tropic 92HT593.1 envelope, and collected 48 h later for flow cytometry analysis. Percentage of productive or latently infected cells after knockdown of the specific protein is shown, with productive infection in green and latent infection in blue. Data shown are means of three independent experiments ± SEM. (D) Scatter plots highlighting the shift in GFP⁺ or BFP⁺ populations after infection of RFP⁺ SRP14 (top) or HMGB3 (bottom) KD Jurkats with DuoFluo virus. Black population represent infected, untransduced Jurkat cells, while green/blue populations represent infected SRP14/HMGB3 KD RFP⁺ Jurkats. Values represent the percentage of GFP⁺ or BFP⁺ cells in KD RFP⁺ Jurkat cells.