FIGURE 3.

SRP14 and HMGB3 knockdown influence tat mRNA splicing. (A) Schematic representation of the NT5C3-Tat splicing reporter construct (Tat+), the corresponding Tat- control and the transactivation reporter cassette (LTR-LucF). The splicing patterns that lead to SA3 activation and Tat expression are indicated. RFP⁺ shRNA KD Jurkats were co-transfected with either the Tat⁺ or Tat– splicing reporter vectors and LTR-LucF, and 24 h later harvested for luciferase readout. (B) The fold changes in Tat splicing (Luciferase Firefly/Renilla ratio for Tat⁺ vs. Tat– cells) over untransduced Jurkat cells were determined. Data represent mean of three independent experiments ± SEM. D, donor; A, acceptor splice site.