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. 2021 Jun 14;12:680725. doi: 10.3389/fgene.2021.680725

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Copyright © 2021 Khoury, Lee, Ramarathinam, McMahon, Purcell, Sonza, Lewin and Purcell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SRP14 expression reactivates latently infected T-cell lines. (A) J-Lat cells were transduced with SRP14-T2A-mtagBFP2 lentivector and cultured with (+Dox) or without (–Dox) doxycycline at 5 μg/mL. Cells were analyzed for mtagBFP2 expression by flow cytometry 72 h post-infection. (B) Histogram depicting EGFP⁺ expression in latently infected T-cells following transduction with SRP14-T2-mtagBFP2. Virus reactivation in J-Lat 10.6, 8.4 and A2 clones is shown by an increase in mean fluorescence intensity (MFI) of BFP⁽⁺⁾EGFP⁽⁺⁾ cells in comparison to EGFP^(–) and EGFP⁽⁺⁾ cells.