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. 2021 Jun 14;9:693694. doi: 10.3389/fcell.2021.693694

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Copyright © 2021 Ying, Zhang, Tu, Chen, Huang, Ying, Wu, Ye, Xiang, Wang, Wang and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The preparation and identification of AAV–5HRE–aFGF–NSCs. (A) The schematic diagrams of the vector construction: AAV–5HRE–aFGF and AAV–5HRE. (B) The immunofluorescence shows the expression of aFGF (red) and DAPI (blue) in different groups. Magnification: 40×; Scale: 50 μm. (C) Western blotting showing the expression of aFGF in each group. (D) The quantitative analysis of aFGF protein expression. **P < 0.01. Data are represented as mean ± SD (n = 6). AAV, adeno-associated virus; 5HRE, five hypoxia-responsive elements; aFGF, acidic fibroblast growth factor; NSCs, neural stem cells.