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. 2021 Jun 14;11:662444. doi: 10.3389/fonc.2021.662444

Figure 3.

Figure 3

siRNAs targeting mouse-specific lncRNA AA388235 activate caspase-3 and increase RNA sensor. (A, B) Effects of Z-DEVD on the cleavage of GSDME and caspase-3 in HCT116 and DLD1 cells transfected with siRNAs as indicated and treated with caspase-3 inhibitor Z-DEVD. GSDME-FL, full-length of GSDME; GSDME-N, the N-terminal cleavage of GSDME; Cleaved-CAS3, the cleavage of caspase-3 p19/p17; cleaved-PARP, the cleavage of PARP. (C, D) Effects of Z-DEVD on siRNAs targeting AA388235-induced pyroptosis, and apoptosis in HCT116 and DLD1 cells. Red arrowheads indicated the pyroptotic cells and yellow arrowheads indicated the apoptosis cells. Scale bar, 100 μm. (E) Immunoblots of MAVS, RIGI, MDA5, TLR3, and P-PKR (phosphor T451) in HCT116 and DLD1 cells transfected with siRNAs as indicated. si-2, siAA388235-2; si-6, siAA388235-6. (F) Immunoblots of caspase-9 in HCT116 and DLD1 cells transfected with siRNAs as indicated. -p37, the cleavage of caspase-9 p37; -p35, the cleavage of caspase-9 p35.