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. 2021 Jun 24;220(9):e202012114. doi: 10.1083/jcb.202012114

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© 2021 Le et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 1. — CYRI-A suppresses cell spreading and leading-edge Arp2/3 complex recruitment.(A) Immunofluorescence images of COS-7 pLKO (control) and CYRI-B CRISPR KO lines (ex3 or ex4.1) expressing either GFP-FLAG or CYRI-A-FLAG vector and stained for Arp2/3 complex (anti-ArpC2, yellow), FLAG-tag (magenta), and F-actin (phalloidin, cyan; scale bar = 20 µm). Dotted square denotes zooms (scale bar = 5 µm). (B and C) Cell spread area and Arp2/3 signal at the leading edge in COS-7 cells expressing either GFP-FLAG (squares) or CYRI-A-FLAG (circles). Data from at least 10 cells per experiment and three independent experiments, each colored separately in blue, orange, and green. Statistical analyses using two-tailed unpaired t test. Mean ± SD. (D) Representative Western blot showing relative expression of GFP-FLAG and CYRI-A-FLAG in COS-7 detected with anti-FLAG antibody. GAPDH as loading control.