Video 2.
CYRI proteins localize to macropinocytic structures prior to RAB5 recruitment. (Seven clips; related to Fig. S2.) Clip 1 (related to Fig. S2 A): Airyscan video of a COS-7 cell expressing P17-GFP-CYRI-B (cyan). Clip 2 (related to Fig. S2 C): Airyscan video of a HEK293T cell expressing P16-GFP-CYRI-A (cyan) and mCherry-RAB5A WT (magenta). Arrowheads indicate the vesicles. Clip 3 (related to Fig. S2 F): Airyscan video of a COS-7 cell expressing P17-GFP-CYRI-B (cyan) and mCherry-RAB5A WT (magenta). Clip 4 (related to Fig. S2 H): Airyscan video of a CHL-1 cell expressing P16-GFP-CYRI-A (cyan) and dextran 70 kD (magenta). Clip 5 (related to Fig. S2 H): Airyscan video of a HEK293T cell expressing P16-GFP-CYRI-A (cyan) and dextran 70 kD (magenta). Clip 6 (related to Fig. S2 N): Airyscan video of a HEK293T cell expressing GFP (cyan) and dextran 70 kD (magenta). Clip 7 (related to Fig. S2 N): Airyscan video of a HEK293T cell expressing P16-GFP-CYRI-A (cyan) and dextran 70 kD (magenta). Acquisition at 9 s/frame and playback at 10 fps.