Video 6.
CYRI-A localization at macropinocytic cups is not abolished by latrunculin-A treatment. (Four clips; related to Fig. 5.) Clip 1 (related to Fig. 5 L): Airyscan video of a COS-7 cell expressing P16-GFP-CYRI-A (cyan) and LifeAct-RFP (magenta) and treated with 1 µM of Latrunculin A (LatA). Acquisition at 30 s/frame and playback at 7 fps. Clip 2 (related to Fig. 5 L): Airyscan video of HEK293T cells expressing P16-GFP-CYRI-A (cyan) and LifeAct-RFP (magenta) and treated with 1 µM of LatA. Acquisition at 30 s/frame and playback at 7 fps. Clip 3 (related to Fig. 5 L): Airyscan video of a zoom of a COS-7 cell expressing P16-GFP-CYRI-A (cyan) and LifeAct-RFP (magenta) and treated with 0.2 mg/ml dextran 70 kD and 1 µM of LatA. Arrows indicate CYRI-A–positive vesicles that contain dextran. Acquisition at 30 s/frame and playback at 2 fps. Clip 4 (related to Fig. 5 L): Airyscan video of a zoom of a HEK293T cell expressing P16-GFP-CYRI-A (cyan) and LifeAct-RFP (magenta) and treated with 0.2 mg/ml dextran 70 kD and 1 µM of LatA. Arrows indicate CYRI-A–positive vesicles that contain dextran. Acquisition at 30 s/frame and playback at 2 fps.