Video 7.
CYRI-A recruitment coincides with and depends on active RAC1 recruitment to macropinocytic structures. (Four clips; related to Fig. 6.) Clip 1 (related to Fig. 6 A): Airyscan video of a HEK293T cell expressing P16-mCherry-CYRI-A (cyan) and GFP-RAC1 WT (magenta). Arrowheads indicate the vesicles. Clip 2 (related to Fig. 6 D): Airyscan video of a HEK293T cell expressing P16-mCherry-CYRI-A (cyan) and CFP-PBD (magenta). Arrowheas indicate the vesicle. Clip 3 (related to Fig. 6 F): Airyscan video of a HEK293T cell expressing P16-GFP-CYRI-A WT (cyan) and P16-mCherry-CYRI-A WT (magenta). Clip 4 (related to Fig. 6 I): Airyscan video of a HEK293T cell expressing P16-GFP-CYRI-A WT (cyan) and P16-mCherry-CYRI-A RRDD (magenta). Acquisition at 9 s/frame and playback at 10 fps.