Video 8.
CYRI-A recruitment to macropinocytic structures depends on PI3-kinase. (Four clips; related to Fig. 7.) Clip 1 (related to Fig. 7 A): Airyscan video of a HEK293T cell expressing P16-mCherry-CYRI-A (cyan) and GFP-GRP1 (magenta). Arrowheads indicate the vesicle. Acquisition at 9 s/frame and playback at 10 fps. Clip 2 (related to Fig. 7 D): Airyscan video of a HEK293T cell expressing P16-mCherry-CYRI-A (cyan) and GFP-Btk (magenta). Arrowheads indicate the vesicle. Acquisition at 9 s/frame and playback at 10 fps. Clip 3 (related to Fig. 7 G): Example 1 of an Airyscan video of a COS-7 cell expressing P16-mCherry-CYRI-A (cyan) and treated with 20 µM LY294002 (as indicated). Arrowheads indicate the vesicles. Acquisition at 30 s/frame and playback at 7 fps. Clip 4 (related to Fig. 7 G): Example 2 of an Airyscan video of a COS-7 cell expressing P16-mCherry-CYRI-A (cyan) and treated with 20 µM LY294002 (as indicated). Arrowheads indicate the vesicles. Acquisition at 30 s/frame and playback at 7 fps.