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. 2021 Jun 23;220(9):e202010177. doi: 10.1083/jcb.202010177

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© 2021 Lv et al.

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Figure S2. — Arih2, Maea, Mgrn1, or Wwp1 do not regulate the ciliary localization of Gpr161.(A) Immunofluorescence showing the cilia (Arl13b, green), Gpr161 (red), and nuclei (DAPI, blue) in WT MEF, GreenBomb, GreenBomb Ift27^−/−, GreenBomb Arih2^−/−, GreenBomb Maea2^−/−, GreenBomb Mgrn1^−/−, or GreenBomb Wwp1^−/−. Scale bar, 5 microns. (B) Quantitation of Gpr161-positive cilia described in A. n = 6 repeats with at least 200 cilia counted per experiment. ****, P < 0.0001 as compared with serum-starved cells by two-way ANOVA. Error bars indicate SD.