Table 1.
Inhibitory effects of HEJG on eight major P450s and two CESs in HLMs (with 3 min or with 33 min preincubation).
| Probe reaction | Target P450 | Time-dependent inhibition IC50 (μg/mL) | Ratio | |
|---|---|---|---|---|
| Preincubation for 3 min | Preincubation for 33 min | |||
| Phenacetin O-deethylation | CYP1A | 287.4 ± 31.5 | 276.2 ± 27.8 | 1.04 |
| Coumarin 7-hydroxylation | CYP2A6 | 750.2 ± 94.8 | 664.9 ± 62.6 | 1.13 |
| Paclitaxel 6α-hydroxylation | CYP2C8 | 312.6 ± 2.8 | 315.2 ± 16.6 | 0.99 |
| Diclofenac 4′-hydroxylation | CYP2C9 | 621.4 ± 55.1 | 568.0 ± 47.4 | 1.09 |
| Omeprazole 5-hydroxylation | CYP2C19 | 614.5 ± 56.7 | 272.0 ± 10.2 | 2.26 |
| Dextromethorphan O-demethylation | CYP2D6 | 2336.0 ± 333.2 | 2484.0 ± 372.0 | 0.94 |
| Chlorzoxazone 6-hydroxylation | CYP2E1 | 810.0 ± 154.4 | 868.8 ± 123.4 | 0.93 |
| Testosterone 6β-hydroxylation | CYP3A | 756.9 ± 61.3 | 191.3 ± 7.7 | 3.96 |
| DME hydrolysis | hCES1A | 717.5 ± 83.6 | 987.5 ± 123.2 | 0.73 |
| NCEN hydrolysis | hCES2A | 219.0 ± 19.9 | 203.8 ± 21.1 | 1.07 |