Table 4.
The inhibitory effects of the chalcones in Radix Glycyrrhizae (Gancao) and the major constituents in Folium Llicis Purpureae (Sijiqing) on CYP3A in HLMs (with 3 min or with 33 min preincubation).
| Compounds | Content (μg/g) | Structures | Time-dependent inhibition IC50 (μM) | Ratio | |
|---|---|---|---|---|---|
| Preincubation for 3 min | Preincubation for 33 min | ||||
| Licochalcone A | 12 |  |
2.00 ± 0.10 | 0.93 ± 0.04 | 2.15 |
| Licochalcone B | 119 |  |
14.07 ± 1.55 | 6.81 ± 0.66 | 2.07 |
| Licochalcone C | 4 |  |
6.02 ± 0.41 | 2.51 ± 0.12 | 2.40 |
| Licochalcone D | 11 |  |
2.18 ± 0.40 | 1.32 ± 0.11 | 1.65 |
| Echinatin | 29 |  |
25.86 ± 3.19 | 8.08 ± 0.56 | 3.20 |
| Kaempferol | 115 |  |
13.12 ± 1.29 | 6.53 ± 0.31 | 2.01 |
| Quercetin | 148 |  |
6.84 ± 0.55 | 3.95 ± 0.31 | 1.73 |
| Rutinum | 1788 |  |
>100 | >100 | – |
| Neochlorogenic acid | 328 |  |
>100 | >100 | – |
| Chlorogenic acid | 1129 |  |
>100 | >100 | – |
| Caffeic acid | 149 |  |
>100 | >100 | – |
| Isochlorogenic acid A | 236 |  |
>100 | >100 | – |
| Isochlorogenic acid B | 217 |  |
>100 | >100 | – |
| Isochlorogenic acid C | 348 |  |
>100 | >100 | – |
| Cryptochlorogenic acid | 360 |  |
>100 | >100 | – |
Data are expressed as mean ± SD (n = 3).