Extended Data Fig. 5 ∣. ELF5 binds to multiple genomic loci of FBXW7 in normal mammary epithelial cells and ELF5 KD MX1 cells show increased expression of IFNGR1.

a, In silico ChIP-seq analysis showing multiple binding sites of ELF5 on genomic loci of FBXW7. Lac1 denotes mammary gland obtained from lactating mouse and P13 denotes mammary gland obtained from pregnant mouse at day 13. Black vertical lines show binding sites. b, FACS analysis showing increase in IFNGR1 protein expression upon stable knockdown of ELF5 in MX1 TNBC cells using multiple shRNAs. Data is representative of two independent experiments. c, d IHC images showing FoxP3 staining in periphery and core of tumor sections obtained from C3-T⁺; ELF5^+/+ and C3-T⁺; ELF5^+/− mice. n=3 samples were used. e, Contour plots and f, g, scatter plots showing increased Ly6G⁺ neutrophil population in tumors from C3-T⁺; Elf5^+/− mice as compared to C3-T⁺; Elf5^+/+ tumors, n=3, C3-T⁺; Elf5^+/+ tumors and n=5, C3-T⁺; Elf5^+/− individual tumors. h, Suppression of T-cell proliferation confirms that increased myeloid cells in tumors are Gr1⁺ myeloid cells. Tumor CD45⁺CD11b⁺Gr1⁺ neutrophils were used. Tumor neutrophils (Gr1⁺ myeloid cells) were enriched using Gr1 enrichment kit following manufacture′s protocol. Data is representative of two independent experiments. (f, g) Mann-Whitney U test was used to calculate statistical significance. Data presented as mean ± SEM. Scale bar, 200 μM.