Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 22;62(7):22. doi: 10.1167/iovs.62.7.22

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2021 The Authors

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

PMC Copyright notice

Figure 6. — Assays for mitochondrial ROS production. (A) Ratio of geometric mean intensity between levels of ROS generation in the vital cells. The rates of production in ROS from four cell lines were analyzed using a BD LSR II flow cytometer system with MitoSOX Red reagent (B) The relative ratio of intensity was calculated. The averages of three independent determinations for each cell line are shown. (C) Western blot analysis of antioxidative enzymes SOD2, SOD1, and catalase in six cell lines with GAPDH as a loading control. (D) Quantification of SOD2, SOD1, and catalase. Average relative values of SOD2, SOD1, and catalase were normalized to the average values of β-actin in various cell lines. The values for the latter are expressed as percentages of the average values for control cell line C1. The averages of three independent determinations for each cell line are shown. Graph details and symbols are explained in the legend to Figure 4.