Table 1.

Effect of selected viral variations on RBD stability and ACE2 binding.

Viral variation	ΔΔG	RBD Stability	ACE2-RBD Binding energy (kcal/mol)	ACE2 binding affinity
N501Y	0.53	Increased	−67.86	High
Y453F	−0.5	Reduced	−60.9	High
G446D	−4.12	Reduced	−55.95	Low
G446V	−2.64	Reduced	−60.16	High
L455F	−0.09	Reduced	−61.61	High
A475V	−0.12	Reduced	−61.19	High
K417N	−1.34	Reduced	−51.25	High
F486I	0.31	Increased	−58.55	Low
F486L	0.38	Increased	−58.09	Low
N501S	0.17	Increased	−61.04	High
N501I	0.8	Increased	−61.69	High
N501T	−0.01	Reduced	−62.15	High
Y505C	−0.06	Reduced	−52.76	Low
Q493L	0.66	Increased	−60.06	High
Q493K	−0.04	Reduced	−56.58	Low
G446S	−4.11	Reduced	−60.21	High
T500S	−0.15	Reduced	−55.72	Low
Y505H	0.09	Increased	−57.21	Low
K417R	0.3	Increased	−62.04	High
Y495F	−0.12	Reduced	−59.86	High
Y449F	0.02	Increased	−55.3	Low
F456L	−0.2	Reduced	−57.33	Low
F456Y	0.26	Increased	−59.55	Low
Y489H	−0.78	Reduced	−57.04	Low
Q493H	0.29	Increased	−60.28	high