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BMSCs are transfected with lentivirus carrying siRNA-Wnt3a. (A) Western blotting of the protein expression level of Wnt3a. (B) Quantitative polymerase chain reaction was used to detect the RNA expression level of Wnt3a. (C) Immunofluorescence was used to detect the expression level of Wnt3a. ***P<0.001 vs. the EV group; ###P<0.001 vs. the normal group. BMSCs, bone marrow mesenchymal stem cells; si, small interfering; EV, empty vector.
