Table 3.
Inhibition rate (%) by l-menthol of binding of a ligand against a specific molecule (a) Ligands of which inhibition rate were more than 0 % and less than 1 %. (b) Ligands of which inhibition rate were 0 %.
| Molecule of interest | Radioactive isotope-labelled ligand | Preparation containing molecule of interest | Inhibition by l-Menthol (%) | Inhibition by a positive substance (%) | Positive substance |
|---|---|---|---|---|---|
|
(a) 1 %>Inhibition >0 % | |||||
| Neuropeptide Y1 receptor (Human) | [125I]-Peptide YY (Porcine) | Human receptor (Non-recombinant) | 0.86 | 99.75 | [Leu 31, Pro 34]-Neuropeptide Y porcine |
| Estrogen receptor | [3H]-Estradiol | Rat uterus | 0.84 | 100 | β-Estradiol |
| Adenosine transporter (Human) | [3H]-S-(p-Nitrobenzyl)-6-thioinosine (3H-NBTI) | Human Receptor | 0.68 | 100 | S-(4-Nitrobenzyl)-6-thioinosine (NBTI) |
| Prostanoid EP2 receptor (Human) | [3H]-Prostaglandin E2 | Human recombinant | 0.66 | 100 | Prostaglandin E2 |
| Muscarinic M2 receptor (Human) | [3H]-Scopolamine Methyl Chloride | Human recombinant | 0.37 | 99.97 | Atropine sulfate salt monohydrate |
| Opiate δ receptor (Human) | [3H]-Naltrindole | Human recombinant | 0.3 | 100 | Naltriben methanesulfonate hydrate |
| GABA A receptor (Agonist site) | [3H]-Muscimol | Rat cerebellum | 0.2 | 99.04 | Muscimol |
| Glutamate receptor (Kainate) | [3H]-Kainic Acid | Rat whole brain | 0.14 | 100 | Kainic acid monohydrate |
| IP3 receptor |
D-[3H]-Inositol-1,4,5-Triphosphate |
Rat cerebellum |
0.12 |
100 |
D-myo-Inositol 1,4,5-trisphosphate potassium salt |
|
(b) Inhibition = 0 % | |||||
| β1-Adrenergic receptor (Human) | [3H]-(-)-CGP-12177 | Human recombinant | 0 | 95.06 | (±)-Propranolol hydrochloride |
| β2-Adrenergic receptor (Human) | [3H]-(-)-CGP-12177 | Human recombinant | 0 | 99.74 | (±)-Propranolol hydrochloride |
| Angiotensin AT2 receptor (Human) | [125I]-CGP-42112A | Human recombinant | 0 | 100 | Angiotensin II human |
| Ca channel (Type L, Benzothiazepine) | [3H]-(+)-cis-Diltiazem | Rat cerebral cortex | 0 | 96.98 | (+)-cis-Diltiazem hydrochloride |
| Ca Channel (Type N) | [125I]-ω-Conotoxin GVIA | Rat whole brain | 0 | 100 | ω-Conotoxin GVIA |
| CCK A receptor (Human) | [125I]-Cholecystokinin Octapeptide | Human recombinant | 0 | 99.47 | CCK-Octapeptide (26–33) (Sulfated Form) (CCK-8) |
| CRF1 receptor (Human) | [125I]-Corticotropin Releasing Factor (Ovine) | Human recombinant | 0 | 100 | Urocortin human |
| Dopamien D1 receptor (Human) | [3H]–SCH–23390 hydrochloride | Human recombinant | 0 | 100 | R (+)–SCH–23390 hydrochroride |
| Dopamine D2 receptor short isoform (Human) | [3H]-Spiperone | Human recombinant | 0 | 94.16 | (+)-Butaclamol hydrochloride |
| Dopamine D5 receptor (Human) | [3H]SCH 23390 | Human recombinant | 0 | 98.18 | R (+)–SCH–23390 hydrochloride |
| Endothelin ETA receptor (Human) | [125I]-Endothelin-1 (Human, Porcine) | Human recombinant | 0 | 100 | Endothelin-1(Human) |
| Glucocorticoid receptor (Human) | [3H]-Dexamethasone | Human recombinant | 0 | 99.36 | Dexamethasone |
| Glutamate receptor (AMPA) | D,L-alpha-[3H]-Amino-3-Hydroxy-Methylisoxazole-4-Propionic Acid (3H-AMPA) | Rat cerebral cortex | 0 | 100 | (S)-AMPA |
| Glutamate receptor (NMDA agonist site) | [3H]-CGP-39653 | Rat cerebral cortex | 0 | 100 | L-Glutamic acid hydrochloride |
| Glycine receptor (Strychnine sensitive) | [3H]-Strychnine | Rat spinal cord | 0 | 98.79 | Strychnine |
| Histamine H1 receptor (Human) | [3H]-Pyrilamine | Human recombinant | 0 | 100 | Pyrilamine maleate salt |
| Histamine H3 receptor (Human) | N-alpha-[3H]-Methylhistamine, Dihydrochloride | Human recombinant | 0 | 100 | (R) (−)-α-Methylhistamine dihydrochloride |
| Leukotriene D4 receptor | [3H]-Leukotriene D4 | Guinea pig lung | 0 | 96.63 | Leukotriene D4 |
| Muscarinic M1 receptor (Human) | [3H]-Scopolamine Methyl Chloride | Human recombinant | 0 | 99.24 | Atropine sulfate salt monohydrate |
| Muscarinic M3 receptor (Human) | [3H]-Scopolamine Methyl Chloride | Human recombinant | 0 | 99.82 | Atropine sulfate salt monohydrate |
| Muscarinic M4 receptor (Human) | [3H]-Scopolamine Methyl Chloride | Human recombinant | 0 | 100 | Atropine sulfate salt monohydrate |
| Muscarinic M5 receptor (Human) | [3H]-Scopolamine Methyl Chloride | Human recombinant | 0 | 99.82 | Atropine sulfate salt monohydrate |
| Neuropeptide Y2 receptor (Human) | [125I]-Peptide YY (Human) | Human receptor (Non-recombinant) | 0 | 98.66 | Neuropeptide Y human |
| Neurotensin NT1 receptor (Human) | [125I]-Neurotensin | Human recombinant | 0 | 99.56 | Neurotensin |
| Nicotinic receptor (Human) | [3H]-(±)-Epibatidine | Human receptor (Non-recombinant) | 0 | 100 | (±)-Epibatidine dihydrochloride hydrate |
| Opiate μ receptor (Human) | [3H]-Diprenorphine | Human recombinant | 0 | 100 | [D-Ala 2, N–Me-Phe 4, Gly5-ol]-Enkephalin acetate salt (DAMGO) |
| PAF receptor | 1-O-Hexadecyl-[3H]-Platelet Activating Factor (3H-PAF) | Rabbit platelet | 0 | 100 | 1-O-Palmityl-sn-glycero-3-phosphocholine (PAF) |
| Serotonin 5HT1A receptor (Human) | [3H]-8-Hydreoxy-DPAT | Human recombinant | 0 | 99.67 | Serotonin hydrochloride |
| Serotonin transporter (Human) | [3H]-Imipramine hydrochloride | Human recombinant | 0 | 96.87 | Imipramine hydrochloride |
| Testosterone receptor (Human) | [3H]-Methyltrienolone (3H-R1881) | Human receptor | 0 | 97.18 | Testosterone |
| Vasopressin V1 receptor | [3H]-Vasopressin, 8-L-Arginine | Rat liver | 0 | 98.89 | [Arg 8]-Vasopressin |
| Vasopressin V1B receptor (Human) | [3H]-Vasopressin, 8-L-Arginine | Human recombinant | 0 | 100 | [Arg 8]-Vasopressin |