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. Author manuscript; available in PMC: 2021 Jun 28.
Published in final edited form as: Dev Cell. 2020 Feb 27;52(6):764–778.e4. doi: 10.1016/j.devcel.2020.01.032

Figure 5. Active cell migration of surface epidermal cells during digit separation.

Figure 5.

(A) EM showing the surface epidermis and (B) the IET in E14.5 forelimbs from WT embryos. Yellow dashed line indicates the basement membrane. (C-D) Immunofluorescence staining with the basement membrane marker Laminin-1 (red) and Collagen IV (green). (E) Schematic representation of ex-vivo live imaging of E13.5 embryonic forelimb using light-sheet fluorescence microscopy. (F) Snapshots of tracked cells at the interdigital zone (IDZ) over time (top panel). Higher magnification views of the IDZ with labeled migrating cells (orange and purple) and non-migrating cell (red) (bottom panel).