Figure 6. Fecal Microbiota Transplantation (FMT) regulates STING protein levels in the colon altering intestinal inflammation.

(A-F) WT FMT alleviates intestinal inflammation and STING protein levels in N153s mice. (A) Schematic image illustrating FMT experimental design. WT FMT (aerobic and anaerobic) was administered via oral gavage to N153s mice every other day for 2 weeks. (B) colon length, (C) stool appearance and (D) pathology score of the indicated mice and treatments (*P<0.05, **P<0.01, ***P<0.001. Data are represented as mean ±SEM of n=3–13 from 2 independent experiments). (E) Representative images of H&E-stained colon sections from the indicated mice. (F) Immunoblot analysis of STING and β-actin in colon tissue lysates isolated from WT and N153s mice with the indicated treatments. (G-L) N153s FMT stabilizes STING protein in the colon and promote colitis in WT mice. (G) Schematic image illustrating FMT experimental design. WT mice were treated with full spectrum antibiotic cocktail for 10 days to deplete commensal bacteria, following 2 days of sterile water. WT or N153s FMT was administrated every other day (aerobic and anaerobic) for 7 days with/wo low concentration of DSS (1%). (H) colon length, (I) stool appearance and (J) pathology score of the indicated mice and treatments (*P<0.05,***P<0.001. Data are represented as mean ±SEM of n=2–5 from 2 independent experiments). (K) Representative images of H&E-stained colon sections from the indicated mice. (L) Immunoblot analysis of STING and β-actin in colon tissue lysates isolated from WT and N153s mice with the indicated treatments.