Figure 4.

miR-301a-3p directly targets STAT3. (A) Schematic representation of the binding site of miR-301a-3p in STAT3-WT an in STAT3-MUT. (B) Dual-luciferase reporter assays were used to demonstrate that miR-301b-3p can target STAT3. (C) The transfection efficiency of miR-301a-3p mimic was determined by real-time quantitative PCR. (D) The expression of STAT3 was determined by western blot analysis. The data are expressed as the mean ± SD. ^**P<0.01 and ^***P<0.001, vs. NC or NC mimics. miR, microRNA; STAT, signal transducer and activator of transcription; WT, wild-type; MUT, mutant; NC, negative control.