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Effect of ISO on cardiomyocyte apoptosis in control and ISO1, 3, 7 and 14d groups. (A) TUNEL staining (brown). (B) Quantification of apoptosis rate among the various groups. (C) Protein expression of cleaved caspases-9/caspase-9 and (D) cleaved caspases-3/caspase-3 were detected by western blotting. *P<0.05 and **P<0.01 vs. control group. ISO, isoproterenol.
