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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: FASEB J. 2021 Feb;35(2):e21287. doi: 10.1096/fj.202001504RR

Figure 5. Ceramide profiles in CerS2-downregulated cells.

Figure 5.

(A-E) SKOV3 cells were transfected with 5 nM siRNA for control-2, CerS2, CerS5, or CerS6 for 48 h and then lipids were extracted. Lipid contents (ceramide, sphingomyelin, hexosylceramide, sphingosine-1-phosphate, and sphingosine) were determined by mass spectrometry (n = 4). (F) Lipids were extracted from CRISPR control (C14) and CerS KO (3-6) cells and then ceramide contents were determined by mass spectrometry (n = 3).