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. 2021 Jun 28;19:97. doi: 10.1186/s12958-021-00781-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — SCD test, AO staining for fluorescence microscopy (AO staining 10 × 40). A Sterile patients with mycoplasma infection; B Males with normal fertility. a. large-size dispersion halos; b. medium-size dispersion halos; c. small-size dispersion halos; d. no dispersion halo. The percentages of sperm small-size dispersion halos and no dispersion halo were significantly higher in Group A than those in Group B. On the contrary, the percentage of sperm with large-size dispersion halos was significantly lower in Group A than that in Group B (P < 0.05)