Fig. 3.

The ratios of fluorescence value/OD₆₀₀ of recombinant strain ZM4_EGFP at different temperatures of 24, 30, 36, 40, and 45 °C using a microplate reader (a), and Western blot result of EGFP in ZM4_EGFP cultured at different temperatures of 24, 30, 36, 40, and 45 °C (b). “ZM4, 30 °C” indicated the control condition of wild-type strain ZM4 grown at 30 °C. Control represented wild-type strain ZM4 cultured at 30 °C. M was Pre-dyed SDS-PAGE Standard. One-way ANOVA of the ratios of fluorescence intensity value and OD₆₀₀ of ZM4_GFP at 24, 36, 40, and 45 °C were conducted with 30 °C as a control at 6, 9, 12, and 24 h, respectively, * represents a significant difference (0.01 < P-value < 0.05), ** represents a very significant difference (P-value < 0.01), *** represents P-value < 0.001, **** represents P-value < 0.0001