Table 2.
List of 49 significantly differentially expressed genes between ZM4 cultured at 30 °C and 45 °C
| Gene ID | Product | Ratio | − log10 (P-value) |
|---|---|---|---|
| Upregulated gene in ZM4 cultured at 30 °C compared with 45 °C | |||
| ZMO0005 | Sulfate adenylyltransferase small subunit | 1.35 | 3.24 |
| ZMO0008 | Sulfite reductase hemoprotein beta-component | 1.31 | 2.59 |
| ZMO0374 | Levansucrase | 1.83 | 5.90 |
| ZMO0375 | Levansucrase/invertase | 1.64 | 5.43 |
| ZMO0379 | PBSX family phage terminase large subunit | 1.53 | 5.18 |
| ZMO0395 | Hypothetical protein | 2.20 | 5.52 |
| ZMO0397 | Hypothetical protein | 1.84 | 4.79 |
| ZMO0454 | Formate-tetrahydrofolate ligase | 2.39 | 10.71 |
| ZMO0492 | Nitrogen regulatory protein PII | 3.16 | 11.78 |
| ZMO1117 | Glutamate synthase large subunit | 1.44 | 7.99 |
| ZMO1719 | Fructokinase | 1.73 | 10.52 |
| ZMO1748 | ArsR family transcriptional regulator | 1.36 | 5.73 |
| ZMOp36x011 | Tail sheath protein | 1.82 | 4.51 |
| ZMOp36x012 | Tail tube protein | 1.60 | 4.35 |
| Downregulated gene in ZM4 cultured at 30 °C compared with 45 °C | |||
| ZMO0122 | Uncharacterized protein | − 2.86 | 9.99 |
| ZMO0286 | DUF541 domain-containing protein | − 2.56 | 9.78 |
| ZMO0693 | OsmC family protein | − 1.57 | 8.5 |
| ZMO0740 | General stress protein CsbD | − 3.06 | 8.03 |
| ZMO1113 | FAD-dependent pyridine nucleotide-disulfide oxidoreductase | − 1.62 | 8.34 |
| ZMO1237 | Lactate dehydrogenase | − 2.82 | 9.39 |
| ZMO1522 | TonB-dependent receptor | − 1.01 | 3.88 |
| ZMO1533 | Hypothetical protein | − 3.01 | 12.07 |
| ZMO1754 | Succinate-semialdehyde dehydrogenase SSADH | − 3.95 | 11.33 |
| ZMO1776 | Aminopeptidase N | − 1.97 | 9.64 |
| ZMO1940 | Hypothetical protein | − 4.87 | 7.45 |
| ZMO0003 | Adenylyl-sulfate kinase | − 1.23 | 2.12 |
| ZMO0246 | ATP-dependent protease subunit HslV | − 2.06 | 11.16 |
| ZMO0400 | Hypothetical protein | − 1.17 | 5.94 |
| ZMO0422 | BadM/Rrf2 family transcriptional regulator | − 1.25 | 7.82 |
| ZMO0660 | Chaperone protein DnaK | − 3.52 | 10.65 |
| ZMO0748 | Cysteine synthase | − 3.3 | 9.68 |
| ZMO0989 | Heat-shock protein IbpA | − 8.63 | 15.44 |
| ZMO1062 | Putative phage shock protein pspD | − 2.33 | 8.1 |
| ZMO1063 | Phage shock protein A PspA | − 2.9 | 10.94 |
| ZMO1271 | Siroheme synthase CysG | − 4.29 | 11.34 |
| ZMO1424 | ATP-dependent chaperone ClpB | − 1.82 | 11.22 |
| ZMO1426 | DNA repair protein RadC | − 1.22 | 6.63 |
| ZMO1463 | TonB-dependent receptor | − 1.41 | 4.44 |
| ZMO1586 | Bacterioferritin | − 1.46 | 5.72 |
| ZMO1721 | Glyoxalase/bleomycin resistance protein dioxygenase | − 4.59 | 13.11 |
| ZMO1849 | Uncharacterized protein | − 3.55 | 8.15 |
| ZMO1928 | Chaperonin Cpn10 | − 6.22 | 13.21 |
| ZMO1929 | Chaperonin GroEL | − 6.03 | 12.81 |
| ZMOp33x029 | Azospirillum phage Cd Gp10 family protein | − 2.4 | 7.07 |
| ZMOp33x030 | Hypothetical protein | − 2.45 | 8.57 |
| ZMOp39x009 | Putative partitioning protein ParA ATPase | − 1.39 | 8.45 |
| ZMOp39x010 | Putative partitioning protein ParB | − 1.23 | 6.27 |
| ZMOp39x036 | Hypothetical protein | − 3.79 | 12.36 |
| ZMOp39x037 | Hypothetical protein | − 4.21 | 11.34 |
Ratio is the log2-based expression difference between ZM4 cultured at 30 °C and 45 °C. Gene name with red and blue color font indicates up-regulated and down-regulated at 30 °C compared with 45 °C, respectively. Eleven genes shown in bold font were up-regulated in both high (45 °C) and low temperature (24 °C) conditions than at normal temperatures (30 °C) (Additional file 3: Table S3)