Table 4.
The expression values (log2RPKM) of genes in ZM4 corresponding to 26 genes related to heat tolerance of Z. mobilis TISTR 548 (ATCC 29191) at different temperatures
| ATCC29191 Gene ID | Product | ZM4 Gene ID | log2RPKM | ||||
|---|---|---|---|---|---|---|---|
| 24 °C | 30 °C | 36 °C | 40 °C | 45 °C | |||
| ZZ6_0707 | Glucose sorbosone dehydrogenase | ZMO0558 | 7.20 | 7.16 | 7.09 | 7.15 | 7.22 |
| ZZ6_1376 | 5,10-methylenetetrahydrofolate reductase | ZMO1747 | 6.39 | 6.57 | 7.20 | 7.87 | 6.08** |
| ZZ6_1146 | Glucosamine-fructose-6-phosphate aminotransferase | ZMO0056 | 7.66 | 7.53 | 7.53 | 7.48 | 8.08* |
| ZZ6_0929 | Glycosyl transferase group | ZMO0306 | 6.62 | 6.36 | 6.40 | 6.30 | 5.73* |
| ZZ6_0923 | Phospholipase D/transphosphatidylase | ZMO0314 | 6.41 | 6.42 | 6.47 | 6.50 | 6.74* |
| ZZ6_1551 | Squalene-hopene cyclase | ZMO1548 | 7.19 | 7.25 | 7.34 | 7.45 | 7.98** |
| ZZ6_1046 | MotA/TolQ/ExbB proton channel | ZMO0161 | 7.93 | 8.12 | 7.99 | 7.69 | 6.75** |
| ZZ6_1043 | Protein TolB | ZMO0165 | 8.56 | 8.68 | 8.45 | 8.09 | 7.91** |
| ZZ6_1254 | Protein-export protein | ZMO1897 | 8.88 | 9.14 | 9.24 | 9.13 | 7.49** |
| ZZ6_1477 | Import inner membrane | ZMO1636 | 9.27 | 9.34 | 9.12 | 9.29 | 10.89**** |
| ZZ6_0158 | Protein of unknown function | ZMO1173 | 6.85 | 7.04 | 7.19 | 7.33 | 6.78* |
| ZZ6_1210 | ComEC/Rec2-related protein | ZMO1965 | 4.84 | 5.01 | 5.17 | 5.18 | 5.52** |
| ZZ6_0840 | MJ0042 family finger-like | ZMO0414 | 8.30 | 8.36 | 8.35 | 8.26 | 7.00*** |
| ZZ6_0541 | Protein of unknown function | ZMO0746 | 7.47 | 7.68 | 7.70 | 7.89 | 7.56 |
| ZZ6_1289 | Protein of unknown function | ZMO1860 | 7.12 | 7.14 | 7.06 | 7.02 | 6.27*** |
| ZZ6_0616 | DNA repair protein | ZMO0663 | 5.67 | 5.66 | 5.63 | 5.82 | 5.67 |
| ZZ6_0934 | Exodeoxyribonuclease 7 large | ZMO0300 | 6.50 | 6.68 | 6.71 | 6.82 | 7.39** |
| ZZ6_0681 | DNA repair protein | ZMO0589 | 6.70 | 6.81 | 6.68 | 6.68 | 6.26* |
| ZZ6_0023 | tRNA/rRNA methyltransferase | ZMO1328 | 7.85 | 8.08 | 8.21 | 8.19 | 7.19* |
| ZZ6_1659 | Peptidase M16 domain | ZMO1422 | 7.74 | 7.46 | 7.23 | 7.20 | 6.67** |
| ZZ6_0980 | Protease Do | ZMO0234 | 8.97 | 8.74 | 8.56 | 8.57 | 10.08**** |
| ZZ6_0702 | ATP-dependent helicase HrpB | ZMO0565 | 6.20 | 6.50 | 6.69 | 6.87 | 6.81* |
| ZZ6_0979 | ATPase-like, ParA/MinD | ZMO0236 | 7.06 | 7.20 | 7.13 | 7.06 | 8.98*** |
| ZZ6_0019 | Flavoprotein WrbA | ZMO1335 | 7.56 | 7.08 | 7.07 | 7.33 | 9.44**** |
| ZZ6_0962 | Pseudogene | No homologue identified | |||||
| ZZ6_0861 | Hypothetical protein | ZMO0391 | 4.23 | 4.93 | 4.24 | 2.27 | 5.70 |
T-test of ZM4 cultured at 45 °C were conducted with 30 °C as a control, * represents a significant difference (0.01 < P-value < 0.05), ** represents a very significant difference (P-value < 0.01), *** represents P-value < 0.001, **** represents P-value < 0.0001