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. 2021 Jun 28;18:145. doi: 10.1186/s12974-021-02193-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Smek1 is required for indoleamine 2,3-dioxygenase 1 (IDO1) maintenance in the immune system and CNS. a, b IFN-γ⁺ splenic and lymph node CD4⁺ Th1 cells were detected by flow cytometry (n = 7 in both groups) (left panel); data are shown in the bar graph (right panel). c qPCR analysis of the relative expression of T-bet and IFN-γ with and without anti-CD3&CD28 treatment. d Western blot and quantification analyses of the protein levels in splenic mononuclear cells with no stimulation and treatment with LPS and the anti-CD3&CD28 and anti-IFN-γ antibodies. e qPCR analysis of the mRNA level of IDO1 in the EAE brain (n = 9 in control, n = 8 in Smek1^-/+ mice). f qPCR analysis of the mRNA level of IDO1 in the EAE spleen (n = 9 in control, n = 8 in Smek1^-/+ mice). g Western blot and quantification analyses of the IDO1 protein levels in the EAE brain. h Western blot and quantification analyses of IDO1 protein levels in the EAE spleen. i Representative western blot analysis of IDO1 expression level in EAE brain and spleen (n = 8 in each group). Data are presented as the mean ± SEM and were analyzed by the two-sided unpaired t test; *P < 0.05; **P < 0.01; ***P < 0.001