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. 2021 Jun 28;21:195. doi: 10.1186/s12866-021-02254-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — a) Mice survival rate. Priming of the respiratory tract with L.psb (1 × 10⁶ CFU/mouse) or L.rff (6 × 10⁶ CFU/mouse) resulted in survival in response to P. aeruginosa infection (6 × 10⁶ CFU/mouse). Statistical significance: *, p < 0.001 for the L.rff + PAO1 and L.psb + PAO1 groups compared to the control PAO1 group, and p < 0.05 for the L.rff + PAO1 group compared to L.psb + PAO1 group. b) Pulmonary P. aeruginosa burden measured on total lung homogenates. Priming of the respiratory tract with L.psb (2 × 10⁵ CFU/mouse) or L.rff (4 × 10⁶ CFU/mouse) 18 h prior to P. aeruginosa infection (6 × 10⁶ CFU/mouse) enhanced the clearance of P. aeruginosa. Statistical significance: *, p < 0.05 compared to the control PAO1 group