Fig. 5.

Caspase 8 and RIPK3 cooperatively mediate Tak1 deficiency–induced mitochondrial ROS and blockade of intracellular Salmonella growth. (A) BMDMs were incubated with 4OHT or vehicle (ethanol) for 5 d. Relative MFIs of MitoSOX in live cells relative to vehicle-treated BMDMs are shown. (B) Representative flow cytometric data in A. (C) Tak1^iKO Casp8^iKO Ripk3^−/− BMDMs were incubated with 4OHT or vehicle for 5 d. Mitochondrial fragmentation was quantified as described in Fig. 2B. (D–F) BMDMs were infected with Salmonella (MOI 10) for 30 min. Extracellular Salmonella was killed by gentamicin. Relative MFIs of MitoSOX in live cells relative to vehicle-treated BMDMs at 18 h post infection are shown (left graph). Intracellular Salmonella numbers were determined at 2 h and 18 h post infection (middle and right graphs). Representative Salmonella colonies are shown (Right). One-way ANOVA, multiple comparisons, and Tukey test; N.S., not significant.