Fig. 3.

PCBP1 regulates Cdkn1A and CD81 transcript levels. (A–C) Induction of Cdkn1A and CD81 transcripts in PCBP1-deleted mouse livers were determined by RNA sequencing, expressed as reads per kilobase of transcript per million reads mapped. (D–G) RNA-binding activity of PCBP1 regulates Cdkn1A and CD81 transcript levels. Tetracycline-inducible stable cell lines expressing EV (−) or PCBP1 variants were transfected with NT or 3′UTR-PCBP1 (P1) siRNA and variant expression induced for 72 h. (D) Protein levels were analyzed by immunoblot using antibodies against PCBP1 and GAPDH. The transcripts’ abundance of Pcbp1 (E), Cdkn1A (F), and CD81 (G) were analyzed by real-time PCR (RT-PCR), normalized to GAPDH, and are shown as relative to NT-controlled siRNA-treated cells. Data are means ± SD of n = 3 or 4 independent experiments. Significant P values, as determined by one-way ANOVA and unpaired t tests with Welch’s correction, are shown. ns, not significant.