Fig. 4.

Depletion of PCBP1 alters cell cycle progression, induces DNA damage, and reduces cell viability. (A and B) Depletion of PCBP1 affects cell cycle progression. HEK293 cells were transfected with NT or PCBP1 (P1) siRNA for 48 h. Cells were fixed, stained, and analyzed by flow cytometry. Histogram showing sub-G1, G0/G1, S, and G2/M phase distribution (A) and quantitation (B) based on DNA content. (C and D) Depletion of PCBP1 induces DNA damage in cells. HEK293 cells were transfected with NT control or PCBP1 (∆PCBP1) siRNA for 48 h. Cells were fixed stained with TUNEL/propidium iodide (PI). (C) Representative image of flow cytometric dot plot analyses of cells after TUNEL/PI staining. Q1–Q4, quadrant 1–4. (D) Composite levels of TUNEL+ cells. (E) Depletion of PCBP1 reduces cell viability. HEK293 cells were transfected with NT or PCBP1 siRNA for 72 h. Cell viability was assessed using cell counting kit eight assays in duplicates. All data are means ± SD of n = 3 to 4 independent experiments. Significant P values, as determined by two-way ANOVA and Šídák’s multiple comparisons test, are shown in B. ****P < 0.0001, ns, not significant. For D and E, P values were determined by unpaired t test with Welch’s correction.