Skip to main content
. 2021 Jun 17;118(25):e2106393118. doi: 10.1073/pnas.2106393118

Fig. 3.

Fig. 3.

Rtt105 promotes RPA nuclear import and its loading at DSBs. (A and B) ChIP-qPCR analysis of the loading of Rfa1-3xFLAG or Rad51-3xFLAG at indicated locations 4 h following DSB induction in the WT or rtt105Δ cells. The positions of the HO cut and the qPCR primers are indicated. (C) Microscopy analysis of Rfa1-YFP subcellular localization in indicated cells. Nup49-mCherry was used to mark the nuclear membrane. (D and E) ChIP analysis of Rfa1-3xFLAG or Rad51-3xFLAG recruitment at indicated locations 4 h after DSB induction in indicated strains. (F) Survival rate of the repair by ectopic recombination in indicated strains. (G and H) ChIP analysis of Rtt105-3xFLAG recruitment 4 h following DSB induction at indicated locations or in indicated strains. The error bar denotes the SD from three independent experiments. **P < 0.01 (Student’s t test).