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. 2021 Jun 17;118(25):e2106393118. doi: 10.1073/pnas.2106393118

Fig. 4.

Fig. 4.

Identification of the RPA residue that mediates the interaction with Rtt105. (A) Graph showing the full-length and truncated Rfa1. The key motifs in Rfa1 are indicated. (B and D) GST pull-down assay showing the interaction between GST-Rtt105 and 6xHis-tagged full-length, truncated, or mutated Rfa1. The amount of GST-tagged proteins was indicated by Coomassie blue staining. (C) GST pull-down assay examining the association between 6xHis-Rtt105 and truncated GST-Rfa1 proteins. The amount of GST-tagged proteins was indicated by Coomassie blue staining. * denotes degraded protein fragments. The bracket marks truncated GST-Rfa1 proteins. (E) Immunoprecipitation analysis the interaction between Rtt105-3xHA and the WT or mutated Rfa1-3xFLAG. (F) A model showing the structure of the F domain of yeast Rfa1, adapted from Deshpande et al. (64). The initiation residue V4, the last residue L134, and the location of V106 are marked.