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. 2021 Jun 14;118(25):e2023752118. doi: 10.1073/pnas.2023752118

Fig. 3.

Fig. 3.

Exposure to 39 °C improves T cell metabolism and function in vivo. (A) Naïve OT-I T cells were injected (i.v) into congenic hosts, and then mice were injected s.c. in the hind flanks with OVA peptide emulsified in IFA. Mice were then either subjected to WBH to raise their temperature to 39 °C or were treated to CTRL at AT. After 8 h, all mice were housed at AT, and then cells were harvested from the mice at the 24-h timepoint. (B) Activation state of donor OT-I cells measured as percent CD69+. (C) Cells from the iLN were incubated ex vivo in the presence of 2-NBDG to measure glucose uptake. (D) Cells from the iLN were incubated with MitoTracker Green to assess mitochondrial mass. (A–D) Data from two independent experiments, n = 7 mice/group. (E) To induce a polyclonal T cell response, anti-CD3/CD28 was injected into the hind footpad of mice. Mice were then either subjected to WBH for 8 h or treated to CTRL at AT. Cells were harvested from the mice 24 h after anti-CD3/CD28 injection. (F) CD8+ cells were isolated from the popliteal LN and (G) iLN , and the mitochondrial mass was measured by MitoTracker Green. Data from two independent experiments, n = 6 mice/group. (H) Western blot of mitochondrial respiratory chain components in CD8+ T cells isolated from iLN 24 h after injection of anti-CD3/CD28 (each column represents cells pooled from two mice). (I) Representative histogram of MTCO1 expression in CD8+ T cells from iLN 24 h after injection of anti-CD3/CD28 measured by flow cytometry (each condition represents cells pooled from three mice). (J) OCR and (K) ECAR at baseline and following administration of oligomycin (oligo), fluoro-carbonyl cyanide phenylhydrazone (FCCP), and rotenone and antimycin (R/A) of CD8+ T cell from iLN 24 h after activation (each group represents cells pooled from three mice). Data from one of two independent experiments. (L) To model ML, irradiated recipient mice were injected with allogeneic BM cells and leukemic (ML: WEHI 3B) cells. After 2 d, 37 or 39 °C TE cells were injected, and survival was monitored. (M). Survival of mice following BMT and leukemia induction. Data from two independent experiments, n = 11 mice/group. **P < 0.01, ***P < 0.001.