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. 2021 Jun 14;118(25):e2022495118. doi: 10.1073/pnas.2022495118

Fig. 2.

Fig. 2.

MCT1 inhibition leads to growth arrest and lactate buildup in early EBV-infected B cells. (A) Bulk PBMCs (n = 3) were treated with AZD3965 immediately following EBV infection, and CD19+ B cell proliferation was based on CellTrace Violet dilution as determined by flow cytometry 4 d later. Cell counts were bead normalized. (B) Bulk PBMCs (n = 3) were treated at 4 d postinfection and after 3 d in media. CD19+ B cell proliferation assessed at day 7 postinfection as in A. (C) CD19-purified B cells (n = 3) were treated at 4 d postinfection and intracellular lactate assessed 3 d later at day 7 postinfection. (D) Same as B, except proliferation was assessed at 14 d postinfection. (E) Growth curve of LCLs (n = 3) treated with varying concentrations of AZD3965. LCL growth was determined by daily assessment of trypan blue exclusion. Statistical significance determined was by a paired Student’s t test. *P < 0.05, **P < 0.01, and ***P < 0.001.