Fig. 2.

LAP2β expression in Lmnb1^−/− (B1KO) embryos. (A) Immunofluorescence microscopy of Lmnb1^+/+ (WT) and Lmnb1^−/− (B1KO) neurons after staining cells with an antibody against LAP2β (white). Red arrows point to B1KO neurons with low levels of LAP2β and/or abnormally distributed LAP2β along the nuclear rim. (Scale bars, 10 μm.) (B) Transcript levels for Tmpo (the gene for LAP2β) in WT and B1KO neurons. Neurons had been differentiated in culture for 48 h. Transcript levels were normalized to Ppia transcripts. Bar graph shows mean ± SEM; n = 3 independent experiments. ***P < 0.0005; unpaired Student’s t test. (C) Immunofluorescence microscopy of B1KO neurons that expressed a NLS-GFP (green) after staining the cells with an antibody against LAP2β. In the Top row, LAP2β is white; in the Bottom row, LAP2β is red. Yellow arrows point to neurons with a NM rupture. LAP2β levels were low in neurons with a NM rupture. (Scale bars, 10 μm.)