Fig. 2.

Association network including MAST-4 species, associated prokaryotes, and other picoeukaryotes from the Malaspina expedition. Only OTUs with abundances >100 reads and occurrences >15% of the stations were considered in MIC analyses. A filtering strategy was applied to remove indirect (i.e., environmentally driven) and weak associations (Methods). Node size is proportional to the centered log-ratio transformed abundance sum (Methods). (A) Nodes are colored based on taxonomy. Legend: DG, Dino-Group. (B) Node color indicates whether specific OTUs displayed weighted mean temperatures significantly lower or higher than the unweighted mean temperature (24.5 °C), pointing to species with temperature distributions that differ from chance. Note that MAST-4A and both MAST-B/C tend to show co-occurrences with other OTUs that display coherent temperature preferences. N.S, not significant.