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. Author manuscript; available in PMC: 2021 Jun 28.
Published in final edited form as: Cell Chem Biol. 2019 Dec 10;27(3):322–333.e5. doi: 10.1016/j.chembiol.2019.11.011

Figure 4.

Figure 4.

Applying CATNIP to profile AT feedback inhibition. (a) Exemplary dose-response profiles of proteins that interact strongly with CoA. (b) Exemplary dose-response profiles of proteins more weakly with CoA. (c) Scheme for isotopic tracing of metabolic source of the acetate group in ac4C. Heavy (U-13C) glucose or acetate were applied in separate metabolic labeling experiments. Incorporation into ac4C was assessed by mass isotopomer analysis of digested total RNA. (d) The major source of ac4C’s N4-acetyl group is glucose. (e) Disruption of ACLY reduces levels of ac4C in poly(A)-enriched, but not total RNA. Values represent ≥ 3 replicates, analyzed by two-tailed student’s t-test (ns = not significant, *P<0.05, **P<0.01, ***P<0.001).